Consulta: creatorFacets:"Leite,G. B."
Registros recuperados: 3
Data/hora: 18/05/2024 05:18:12

Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations
Provedor de dados: 80
Autores: Bueno,L. G. F.; Leite,G. B.; Cruz-Höfling,M. A.; Rodrigues-Simioni,L.; Oshima-Franco,Y.
In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreated with 0.66 and 1.6mM Mn2+ did not affect Cdt venom-induced blockage nor change KCl-induced contracture but partially reduced ACh-induced contracture. However, both Mn2+ concentrations partially hindered Bjssu venom-induced blockage after washing the preparations with Krebs solution, and only 1.6mM Mn2+ preparations significantly recovered ACh-induced contracture. The effect of Cdt venom myotoxicity on contractile responses was different from that of Bjssu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contracture; Divalent cation; Mn2+; Myotoxicity; Neuromuscular blockage; Neurotoxicity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200007

Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)
Provedor de dados: 80
Autores: Oshima-Franco,Y.; Alves,C. M. V.; Andréo Filho,N.; Gerenutti,M.; Cintra,A. C. O.; Leite,G. B.; Rodrigues-Simioni,L.; Silva,M. G.
Numerous plants are used as snakebite antidotes in Brazilian folk medicine, including Casearia sylvestris Swartz, popularly known as guaçatonga. In this study, we examined the action of a hydroalcoholic extract from C. sylvestris on the neuromuscular blockade caused by bothropstoxin-I (BthTX-I), a myotoxin from Bothrops jararacussu venom, in mouse isolated phrenic nerve-diaphragm (PND) preparations. Aqueous (8 and 12 mg/ml, n=4 and 5, respectively) and hydroalcoholic (12 mg/ml, n=12) extracts of the leaves of C. sylvestris caused facilitation in PND preparations followed by partial neuromuscular blockade. BthTX-I (20 µg/ml, n=4) caused 50% paralysis after 65±15 min (mean ± S.E.M). Preincubation (30 min at 37° C) of BthTX-I (20 µg/ml, n=4) with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative medicine; Phytotherapy; Plant extracts; Snakebite.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400007

Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-I
Provedor de dados: 79
Autores: Oshima-Franco,Y.; Leite,G. B.; Valério,A. A.; Hyslop,S.; Andriao-Escarso,S.; Giglio,J. R.; Prado-Franceschi,J.; Cruz-Höfling,M. A.; Rodrigues-Simioni,L.
Bothrops jararacussu venom and its major toxin bothropstoxin-I (BthTX-I) possess myotoxic and neurotoxic properties. The efficacy of a rabbit antivenom raised against B. jararacussu venom in the neutralization of physiological, biochemical, and morphological changes induced by the venom and its major toxin BthTX-I was studied in mouse isolated phrenic nerve-diaphragm (PND) and extensor digitorum longus (EDL) preparations. The times required for 50% neuromuscular blockade in PND and EDL preparations for venom were 70+11.5 (S.E.M., n=5) min and 58+8 (n=16) (50 mu g/mL), and for BthTX-I 31+6 (n=3) min and 30+3 (n=5) min (20 mu g/mL), respectively. After 120 min incubation, creatine kinase (CK) concentrations in solution containing the EDL preparations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops venom; Myotoxin; Neutralization; Neuromuscular junction; Histological analysis; Bothrops jararacussu.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200004